This is the bulk version of RealHelix™ qPCR Kit [Green].

If you would like to purchase in bulk, please contact sale@nanohelix.net.

RealHelix™ 2x qPCR Premix [Green] is designed to perform a rapid real-time quantification of target DNA using a double strand DNA-binding green fluorescent dye. The convenient 2x premix contains hot-start PCR enzyme, green fluorescent dye, dNTPs, buffers, Mg²⁺, and stabilizing agent. The hot-start PCR enzyme provides high specific amplification of target DNA and minimizes the side products such as primer dimers. The separately supplied ROX dye could be used as a passive reference dye in real-time PCR instruments that are compatible with the evaluation of the ROX signal. Bulk version is premix only. ROX reference dye is available separately upon request.

Figure 1. The accuracy of real-time PCR using RealHelix™ qPCR Kit [Green].

Human genomic DNAs (10, 1, 0.1, and 0.01 ng) isolated from whole blood were analyzed using the alpha 1-Antitrypsin (AAT) DNA-specific primer set and an intercalating dye. The accuracy of real-time PCR is demonstrated by E=101.7 %, R² value=0.999. Performed on the CFX96 real-time PCR detection system (Bio-Rad).

Figure 2. Comparison of real-time PCR using RealHelix™ qPCR Kit [Green] and the previous version (RealHelix™ qPCR Kit [Intercalator type]).

Human genomic DNAs (100, 10, 1, and 0.1 ng) isolated from whole blood were analyzed using a beta-globin gene-specific primer set and an intercalating dye. Performed on the CFX96 real-time PCR detection system (Bio-Rad).

Storage

• Store below -20 ℃
• Should be stored in a dark place.

Shelf life

• 12 months